Table 2. Gene Ontology (GO) analysis of differentially expressed genes with ≥ 2.0 fold change, associated with CWD disease in spleen (SP), retropharyngeal lymph nodes (RPLN) and tonsil (TO) tissues of elk.
| GO Function (Biological and Molecular processes) | Gene ID | Gene Name | SP | RPLN | TO |
|---|---|---|---|---|---|
|
Apoptosis and cell cycle |
Bt.64777 Bt.64827 Bt.2408 Bt.13573 Bt.15980 |
Baculoviral IAP repeat-containing 3 Cadherin 1, E-cadherin (epithelial) Chemokine (C-C motif) ligand 2 CDC28 protein kinase regulatory subunit 2 Cyclin B1 |
2.03 2.72 2.10 2.38 |
|
-2.02 |
| |
Bt.4539 Bt.34390 Bt.4922 Bt.6324 Bt.357 Bt.76113 Bt.87249 |
Angiotensin II receptor, type 1 Cysteine and glycine-rich protein Myosin, light chain 1, alkali Myosin, light chain 9, regulatory S100 calcium binding protein A12 (calgranulin C) S100 calcium binding protein A14 S100 calcium binding protein A9 |
-2.34 2.46 2.11 -3.79 2.69 |
|
-4.86 -2.12 -5.63 |
|
Calcium binding and regulation |
Bt.66812 Bt.59155 Bt.15705 Bt.97 Bt.4057 Bt.120 |
Sarcoglycan, β Cytokeratin 19 Destrin (actin depolymerizing factor) Fatty acid binding protein 4, adipocyte Myosin, heavy chain 10, non-muscle Rho-associated, coiled-coil containing protein kinase 2 |
-2.28 2.21 -3.47 -5.11 -2.15 -2.67 |
|
|
|
Cytoskeleton organization and cytokinesis |
Bt.59726 Bt.16055 Bt.9714 Bt.575 Bt.6141 Bt.45294 Bt.89332 Bt.45158 Bt.62630 Bt.4703 Bt.33253 Bt.64757 Bt.8880 Bt.49324 Bt.74664 Bt.53077 Bt.97350 Bt.8897 Bt.41406 |
Caldesmon 1 Transgelin Actin, gamma 2, smooth muscle, enteric Common salivary protein BSP30 form a Desmin Elongation factor RNA polymerase II Fibroblast growth factor-binding protein Filamin A, α Mal, T-cell differentiation protein 2 Myocilin Myotilin Neurotrophic tyrosine kinase, receptor SPARC-like 1 (mast9, hevin) Stress-induced-phosphoprotein 1 (Hsp70/Hsp90-organizing protein) Tensin 3 Tropomyosin 2 (β) Troponin C type 2 (fast) Von Ebner minor salivary gland protein Zinc finger protein 618 |
-5.00 -3.00 -3.81 -3.91 2.02 -2.17 2.77 2.50 -2.25 -3.45 2.53 -2.84 -3.37 2.02 2.75 |
|
-5.83 -2.57 -2.76 |
|
Immune and inflammatory response |
Bt.91780 Bt.4259 Bt.88495 Bt.9560 Bt.26536 Bt.13542 Bt.68530 Bt.49740 Bt.89770 Bt.29871 Bt.62645 Bt.88760 |
Cathelicidin 1 Cathelicidin 2 Cathelicidin 6 Chemokine (C-C motif) ligand 20 Chemokine (C-C motif) ligand 21 Complement factor B Complement factor H Interleukin 8 Lysozyme (renal amyloidosis) Major histocompatibility complex, class II, DY α MHC class I heavy chain Serum amyloid A-like |
-6.61 -9.89 -2.98 3.29 2.51 -2.78 3.82 2.05 4.51 |
-2.09 |
2.70 -3.03 |
|
Protein folding |
Bt.88837 Bt.21932 |
DnaJ (Hsp40) homolog, subfamily A, member 1 F-box protein 7 |
2.53 2.24 |
2.14 |
|
|
Proteolysis |
Bt.77495 Bt.12553 Bt.13092 |
ADAM-like, decysin 1 Haptoglobin Matrix metallopeptidase 7 |
3.07 4.78 |
3.32 |
|
|
Regulation of transcription |
Bt.22888 Bt.87332 Bt.45673 |
Nuclear receptor subfamily 3, group C Similar to HMGB2 protein Spi-C transcription factor |
-3.05 2.38 -7.14 |
|
|
|
Signal transduction |
Bt.44951 Bt.53744 Bt.3081 Bt.21143 Bt.20059 Bt.3704 Bt.6915 Bt.9632 Bt.61075 |
Intersectin 1 (SH3 domain protein) IQ motif containing GTPase activating protein 1 Membrane-spanning 4-domains Rho GTPase activating protein 29 Ribosomal protein S6 kinase, 70kDa Stathmin-like 2 CD163 molecule Deleted in malignant brain tumors 1 Similar to macrophage receptor MARCO |
-2.19 -2.38 -2.57 -5.46 -2.36 -2.28 |
-2.01 |
2.48 2.01 |
|
Transport |
Bt.49731 Bt.79147 Bt.10591 Bt.45049 Bt.4482 Bt.43781 Bt.14388 Bt.47454 Bt.52764 Bt.22651 |
Carbonic anhydrase II Growth hormone receptor Hemoglobin α chain Hemoglobin, β Macrophage scavenger receptor 1 NECAP endocytosis associated 2 Potassium channel tetramerisation domain containing 15 Potassium voltage-gated channel Proliferating cell nuclear antigen Solute carrier family 39 (zinc transporter) |
-2.22 -2.14 -7.54 -19.6 -3.81 -3.51 2.05 -2.00 |
2.11 |
-2.69 |
|
Cellular process |
Bt.48705 Bt.37052 Bt.28966 Bt.67194 Bt.30108 Bt.37111 Bt.57571 Bt.4844 Bt.42764 Bt.61915 Bt.20277 Bt.58848 Bt.49598 |
GINS complex subunit 4 Hemicentin 1 Hypothetical protein LOC100125947 Lysozyme 1 Meiotic nuclear divisions 1 homolog Microtubule-associated protein 1B PDZ and LIM domain 3 Pleiotrophin Prosaposin Similar to 90-kDa heat shock protein α Topoisomerase (DNA) II α 170kDa Ubiquitin carboxyl-terminal esterase L1 Cysteine-rich, angiogenic inducer, 61 |
2.02 -2.90 4.22 2.68 2.18 -2.55 -2.15 -3.45 -2.14 2.12 2.22 -2.15 |
2.28 2.49 |
|
|
Cell adhesion |
Bt.63411 Bt.44183 Bt.9807 Bt.20373 Bt.121 |
Cadherin 18, type 2 Fibulin 5 Glycoprotein (transmembrane) nmb Neuropilin 1 Frizzled-related protein |
-2.03 -2.69 -2.92 -2.08 -2.21 |
|
|
|
Structure Organization |
Bt.6630 Bt.13391 |
Calponin 1, basic, smooth muscle Capping protein (actin filament) muscle Z-line, α 2 |
-4.60 |
|
-2.03 |
|
Response to stimulus |
Bt.4001 Bt.51574 Bt.8738 Bt.12309 Bt.96882 |
Heme oxygenase (decycling) 1 Caveolin 1, caveolae protein, 22kDa Heat shock 105kDa/110kDa protein 1 Heat shock 70kDa protein 8 Similar to RIKEN cDNA 1110064P04 gene |
-3.81 -2.02 2.10 2.44 |
3.14 |
|
|
Angiogenese and axonogenesis |
Bt.91353 Bt.5240 Bt.40062 Bt.3137 |
Angiopoietin 1 Connective tissue growth factor Amyloid β (A4) precursor protein (peptidase nexin-II, Alzheimer disease) Stathmin 1/oncoprotein 18 |
-2.52 -2.12 2.62 |
2.10 |
|
|
Phosphatase activity |
Bt.20846 |
CTD (C-terminal domain, RNA polymerase II, polypeptide A) |
-2.00 |
|
|