Table 6. Oligonucleotides and probes used for qRT-PCR analysis of the DE genes.
| Gene | Nucleotide sequence (5′-3′) | Probe | Accession number |
|---|---|---|---|
| Synuclein A |
F: GGAGCAGGGAGCATTGCA R: TGCCCATATGATCCTTTTTGC |
CTGCCACTGGCTTT |
NM_001034041 |
| Neuropeptide Y |
F: CGGAGGACTTGGCCAGATAC R: TGCCTGGTGATGAGATTGATG |
ACTCAGCGCTGCGAC |
NM_001014845 |
| Cocaine and amphetamine responsive transcript (CART) |
F:CGCGAGCCCTGGACATC R:GCTTCAATCAGCTCCTTCTCATG |
TCCGCCGTGGAGGA |
NM_001007820 |
| Synaptosomal-associated protein (SNAP25) |
F: CCGTCATATGGCCCTGGAT R: TGTCGATCTGGCGGTTCTG |
TGGGCAATGAGATTGATA |
NM_001076246 |
| Tachykinin 1 (TAC1) |
F: CCGTGGCAGTGATTTTTTTCA R: CGTTGGCTCCGATTTCTTCT |
CTCCACTCAACTGTCTG |
NM_174193 |
| Parvalbumin (PVALB) |
F: CCGGAAGACGTGAAGAAGGTAT R: CTCGATGAAGCCGCTCTTG |
CCACATCCTGGATAAAG |
NM_001076114 |
| Visinin-like 1 (VSNL1) |
F: ATCACCCGAGTGGAGATGCT R: TCACTGTGCCCACCATTTTG |
AGATCATCGAGGCTATC |
NM_174490 |
| Tax-1 Binding protein1 (Tax-1BP1) |
F: TCAGGCAACACGGCAAGAA R: TTATCCCGCACATTTACAGCAT |
CTGTCTTTCTGGCTAAAG |
NM_001046409 |
| Superoxide dismutase (SOD1) |
F: TGGAGACCTGGGCAATGTG R: ACAATATCCACGATGGCAACAC |
CAGCTGACAAAAAC |
NM_174615 |
| Angiotensin II receptor (AGTRL1) |
F: CTCATGAACGTCTTCCCCTACTG R: GGGTTGAGGCAGCTGTTGA |
ACGTGCGTCAGCTAC |
NM_001102524 |
| Chemokine 20 (CCL20) |
F: CCCAGTATTCTTGTGGGCTTCA R: GCATTGATGTCACAGGCTTCA |
ACAGCAGCTGGCC |
NM_004591 |
| Serum amyloid A-like (SAA) |
F: ACTCTGACATGAGAGAAGCCAACTA R: CGCGGGCGTGGAAGT |
AAGGGTGCAGACAAA |
NM_001075260 |
| Bos taurus ribosomal protein L12 (RPL12) | F: AGGGTCTGAGGATTACAGTGAAAC R: GATCAGGGCAGAAGCAGAAGG |
ACCATTCAGAACAGAC | NM_205797 |
Primers were designed using the Primers Express 3.0 software of Applied Biosystems. F: Forward primer; R: Reverse primer.