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. 2012 Jul 1;6(3):261–275. doi: 10.4161/pri.19215

Table 2. Primary antibodies used to characterize cells in the present study.

Antibody Specificity Cellular expression Source References
Mouse anti-ovine MHCII (SBU-II, 28–1)
Pan-MHCII
Dendritic cells, macrophages, B lymphocytes
The University of Melbourne, Melbourne, Australia
63
Mouse anti-bovine CD11c (BAQ153A)
CD11c
Dendritic cells, macrophages
VMRD, Inc., Pullman, WA, USA
64
Mouse anti-bovine CD11b (MCA1425, clone CC126)
CD11b
Macrophages, monocytes, granulocytes
Serotec, Ltd., Oxford, UK
65
Mouse-anti-bovine CD205 (MCA1651)
CD205
Dendritic cells, some T-cells, some B-cells, some epithelial cells
Serotec, Ltd., Oxford, UK
44
Mouse anti-human macrophage (EBM11)
CD68
Macrophages, monocytes
Dako, Glostrup, Denmark
66
Polyclonal rabbit anti-human CD209 (DC-SIGN, AHP627)
CD209/DC-SIGN
Dendritic cells
Serotec, Ltd., Oxford, UK
67
Polyclonal rabbit anti-human CD3 (A 0452)
CD3
All T-cells
Dako, Glostrup, Denmark
68
Mouse anti-ovine B cells (DU2–74–25) CD21 B cells, follicular dendritic cells Kind gift from Dr. W. Hein 69