Figure 1.

Cross-view of microphysiometry cell chamber. A polycarbonate insert containing neurons cultured as described with and without glia were placed in the sensor cup. A spacer and additional insert are placed on top of the cells, and the sensor head lowered over the assembly. The sensor head features inlet and outlet tubes to allow for 100 μL/min flow through the 3 μL chamber, as well as the Pt wires for amperometric detection of glucose, lactate, and oxygen, and a stainless steel counter electrode for the acid-sensitive LAPS electrode. A Ag/AgCl (2 M KCl) reference electrode is placed downstream of the chamber.