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Number of culture positive plates detected using approximately 150 mg neat sputum plug plated directly onto PGCF plates (research) compared to the health protection agency (HPA) standard for mycological investigations (10 μl of homogenized sputum) or microbiological investigations (10 μl of diluted-homogenized sputum) plated onto SC plates, n = 55. **P <0.01, ***P < 0.001, HPA methods compared to research using McNemar test.
