Table 2.

Number of Aspergillus fumigatus colonies isolated from COPD patient sputum plated onto either potato dextrose agar (PGCF) or Sabouraud dextrose agar (SC) at various dilutions, and the time period during which colony growth was fi rst observed.

		Number of A. fumigatus colonies isolated
		diluted-homogenized				Homogenized				Plug
		10 μl		100μl		10μl		100μl		150 mg 1
Sputum Sample	Time period	PGCF	SC	PGCF	SC	PGCF	SC	PGCF	SC	PGCF	SC
1	7 days							1
2	7 days									1
3	4 – 6 days									1
4	4 – 6 days							1		1
5	4 – 6 days										1
6	40 – 48 h					1					1
7	40 – 48 h									1
8	40 – 48 h							1	1	1
9	40 – 48 h									2
10	40 – 48 h									2
11	40 – 48 h									2
12	40 – 48 h									3
13	40 – 48 h							4	1	2	7
14	40 – 48 h							4		28
15	40 – 48 h					3	1	13	11	18	31
16	40 – 48 h					5	20	26	34	>40	>50
% positive (n = 16)		0	0	0	0	19	13	44	25	81	31

¹range 100 – 250 mg.