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. Author manuscript; available in PMC: 2012 Jul 18.
Published in final edited form as: J Immunother. 2008 Nov-Dec;31(9):830–839. doi: 10.1097/CJI.0b013e31818817c5

Figure 1.

Figure 1

Lentiviral vector mediated transduction of PBL. A, left panel: PBL were activated using soluble OKT3 for 3 days before starting transduction by gamma-retroviral or lentiviral vector harboring GFP; right panel: PBL were transduced with gamma-retroviral or lentiviral vector harboring GFP with simultaneous OKT3 activation. GFP was measured by FACS at 2 days post-transduction for 16 days. B, PBL were transduced with lentiviral vector harboring GFP upon bead (anti-CD3/CD28) activation using RetroNectin or spinoculation in the presence of 10 μg/ml protamine sulfate. For RetroNectin, the transduction was conducted using vector coated plates while for spinoculation, PBL were mixed with vector and centrifuged at 1000 g, 32 °C for 2 h. Six days post-transduction, the expression of GFP was measured by FACS.