Table 1.
Differentially Expressed Genes (Cluster 1) in Arabidopsis Leaves after Transfer from 12L/12D Rhythm to Short-Day Conditions.
| AGI code | Fold change | Description | Location | Biological process |
| AT3G27060* | 7,12 | ATTSO2 | Cell cycle | |
| AT1G28160 | 3,69 | Member of the ERF subfamily B-1 of ERF/AP2 transcription factor family | Nucleus | Transcription |
| AT2G40350* | 3,67 | Member of the DREB subfamily A-2 of ERF/AP2 transcription factor family | Regulation of transcription | |
| AT4G30650* | 3,23 | Unknown protein | ||
| AT1G69190 | 2,96 | Bifunctional cytosolic hydroxymethyldihydropterin pyrophosphokinase/dihydropteroate synthase (HPPK/DHPS) | Cytosol | Tetrahydrofolate biosynthesis |
| AT1G28520 | 2,92 | VASCULAR PLANT ONE ZINC FINGER PROTEIN | ||
| AT2G15970* | 2,82 | ARABIDOPSIS THALIANA COLD-REGULATED413 PLASMA MEMBRANE 1 | Plasma membrane, vacuole | |
| AT1G53290 | 2,79 | Galactosyltransferase family protein | Protein glycosylation | |
| AT2G24330 | 2,74 | Unknown protein | ||
| AT2G21660* | 2,70 | COLD, CIRCADIAN RHYTHM, AND RNA BINDING 2 (CCR2) (AtGRP7) | Circadian rhythm | |
| AT3G13500 | 2,64 | Unknown protein | ||
| AT5G15960↑ | 2,62 | Cold and ABA inducible protein kin1 | ||
| AT2G30720 | 2,61 | Thioesterase family protein | ||
| AT2G24290 | 2,38 | Na+- and K+-sensitive 1 | ||
| AT3G26470 | 2,37 | Unknown protein | ||
| AT1G13930* | 2,33 | Involved in response to salt stress | ||
| AT2G35733 | 2,33 | Unknown protein | ||
| AT2G47070 | 2,32 | SQUAMOSA PROMOTER BINDING PROTEIN-LIKE 1 | ||
| AT2G42070 | 2,31 | ARABIDOPSIS THALIANA NUDIX HYDROLASE HOMOLOG 23 | Chloroplast | |
| AT3G26740↓* | 2,26 | CCR-LIKE | Chloroplast | Circadian clock |
| AT1G10760* | 2,13 | STARCH EXCESS1, starch degradation | Chloroplast | Carbohydrate metabolism |
| AT2G40840* | 2,12 | DISPROPORTIONATING ENZYME 2 | Cytosol | Carbohydrate metabolism |
| AT1G20440↑* | 2,10 | COLD-REGULATED 47 Dehydrin | ||
| AT4G11600* | 2,08 | GLUTATHIONE PEROXIDASE 6 | Chloroplast, mitochondria, cytosol | Oxidative stress defense |
| AT4G26820 | 2,06 | Unknown protein | ||
| AT1G05170↑ | 2,04 | Galactosyltransferase family protein | Protein glycosylation | |
| AT1G52870 | 2,04 | Peroxisomal membrane protein-related | ||
| AT3G18080* | 2,04 | B-S GLUCOSIDASE 44 | Cell wall | Carbohydrate metabolism |
| AT5G62350* | 2,04 | Invertase/pectin methylesterase inhibitor family protein | ||
| AT5G01370 | 2,03 | ALC-INTERACTING PROTEIN1 | Nucleus | |
| AT1G20620* | 2,01 | CATALASE 3 | Mitochondrion, peroxisome | Hydrogen peroxide catabolic processes |
| AT5G13930↑ | 0,35 | CHALCONE SYNTHASE | ER | Flavonoid biosynthesis |
Arabidopsis was grown for 2 weeks in 12-h photoperiod and then transferred to 8-h SD photoperiod for 2 d. Gene expression is indicated as a ratio of transcript level in leaves transferred to SD in comparison to leaves before the transfer. The fold-change values are means of three independent biological replicates. ↑↓, genes induced or repressed by high-light treatment, respectively (accession AT-00246 in Genevestigator database, Kleine et al., 2007). * Genes repressed in Arabidopsis shoot apex after transfer of 5-week-old plant from SD to LD photoperiod (see text for details).
Rosette leaves were harvested from plants grown under 100 μmol photons m−2 s−1 at 20°C under 12L/12D for 2 weeks and thereafter transferred to SD (8L/16D) for 2 d. Total RNA was isolated with Trizol reagent and labeled by the aminoallyl method with Cy3 or Cy5 fluorescent dyes. RNA isolation, cDNA synthesis, labeling, hybridization, and the data analysis were performed as described in Lepistö et al. (2009). Genes up-regulated more than twofold or down-regulated more than 0.5-fold with P < 0.10 are shown in the table.