Fig. 2.

(a) Four serial rounds of biopanning resulted in the progressive enrichment of two phage products of approx. 450 and 600 bp respectively (highlighted by arrows) as judged by PCR of total phage after each round. The final round was eluted using either SDS (5a) or E. coli (5b), and resulted in differential enrichment of these two bands (see right hand image for clarity); (b) PCR analysis of single colony isolation of phage from the final round of biopanning (representatives of rounds 5a&b are shown); (c) Sequence analysis of the phage PCR products identified a common insert containing a translated 83 amino acid section (red) of FBP21 including its tandem WW domains (underlined) - the translated sequence for a single clone BV14F is shown alongside that for FBP21 (NM_007187).