Fig. 4.
Enhanced FBP21 expression mitigates the effect of 2 on alternative splicing of VEGF in RPE cells. RPE cells were transfected with either control vector or FBP21 expression vector. (a) ELISA for VEGFxxxb using biotinylated VEGFxxxb specific detection antibody, *p<0.05 compared with control, Paired t test, n=3; (b) Transfected cells were then treated with 0.5μM 2 for 72 h and protein assayed by ELISA; results are expressed relative to vehicle (0.1% DMSO) treated cells, P=0.05 Mann Whitney U test, n=4.