TABLE 3.

Time Course of Increases in Neuropeptide Y Immunoreactivity After Kainic Acid–Induced Status Epilepticus

Region	KA 24 h (n = 9), %	KA 8 d (n = 9), %	KA 1 mo (n = 10), %	KA 3 mo (n = 15), %
Proximal subiculum	99 ± 4.2	149 ± 17.8*	193 ± 18.5†	280 ± 28.1†
Distal subiculum	106 ± 5.1	161 ± 27.7‡	169 ± 22.8†	245 ± 24.6†
Presubiculum	88 ± 5.3	108 ± 2.7	99 ± 5.4	140 ± 18.4†
Parasubiculum	91 ± 5.6	123 ± 9.7	152 ± 13.5*	221 ± 52.9†
Medial EC
Layers II–III	93 ± 3.7	104 ± 3.2	108 ± 5.6	177 ± 30.4†
Layers V–VI	102 ± 6.0	124 ± 8.0*	121 ± 8.1	179 ± 16.9†
Lateral EC
Layers II–III	129 ± 6.4	153 ± 22.7	248 ± 26.3†	316 ± 55.7†
Layers V–VI	105 ± 5.0	152 ± 17.3*	187 ± 18.3†	264 ± 39.3†
Perirhinal cortex	135 ± 8.5	202 ± 39.2*	322 ± 34.3†	378 ± 64.8†

Relative optical density (ROD) values of neuropeptide Y immunoreactivity (NPY-ir) of the respective areas were determined in images of 30-μm-thick sections labeled for NPY-ir using the image processing software ImageJ. Relative optical densities are presented as percent of controls ± SEM.

Animal numbers (n) are given in parenthesis; 22 rats were used as controls.

Statistical analysis was performed by analysis of variance with Dunnett multiple comparison post hoc test for group comparisons

^*p < 0.05;

^†p < 0.001;

^‡p < 0.01.

EC, entorhinal cortex; KA, kainic acid.