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. Author manuscript; available in PMC: 2012 Jul 18.
Published in final edited form as: J Nat Prod. 2012 Jan 24;75(2):167–174. doi: 10.1021/np200722w

Figure 2.

Figure 2

Effects of the O. affinis cyclotide extract on cell proliferation of activated human peripheral blood mononuclear cells. CFSE-labeled primary human PBMC were antibody-activated (anti-CD3/CD28 mAbs) and cultured in the presence of medium (ctrl), camptothecin (CPT, 30 μg/mL), or different concentrations (50–100 μg/mL) of O. affinis cyclotide extract. The cells were further analyzed for cell viability and proliferation capacity using flow forward-side-scatter-based flow cytometric analysis (A and B). Cell division analyses were assessed by FACS and illustrated as representative dot plots (C). Results are summarized from three independent experiments in (D), and data are presented as mean ± SEM.