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. Author manuscript; available in PMC: 2012 Jul 18.
Published in final edited form as: J Nat Prod. 2012 Jan 24;75(2):167–174. doi: 10.1021/np200722w

Figure 5.

Figure 5

Effects of kalata B1 on cytotoxicity of activated human peripheral blood mononuclear cells. Human primary PBMC were activated with anti-CD3/CD28 mAbs in the presence of medium (ctrl), camptothecin (CPT, 30 μg/mL), Triton-X 100 (T-x), or different concentrations of kalata B1 (1.8–14 μM) and analyzed for “subG1” DNA content (A) by flow cytometry. Cells were stained with annexin V and propidium iodide (PI) to assess the percentages of viable (annexin V−/PI−), apoptotic (annexin V+/PI− or annexin V+/PI+), and necrotic (annexin V−/PI+) cells. Dot plots were analyzed, and representative graphs are shown (B). Results from three independent experiments are summarized, and data are presented as mean ± SEM (C and D). n.d. = not detectable.