Table 4.
Mean ± SEM number of c-Fos Immunoreactive Cells in the Regions with Significant Differences between the Vehicle (n = 8), 1 mg / kg Oxytocin (n = 8) and 100 mg / kg WAY 267,464 (n = 8) Conditions.
Region (Fig. 1) | Bregma | Vehicle | 1 mg/ kg Oxytocin |
100 mg / kg WAY 267,464 |
---|---|---|---|---|
Areas with significant WAY 267,464- and oxytocin-induced c-Fos expression above vehicle |
||||
18. Paraventricular hypothalamic nucleus | −1.80 | 8.7 ± 2.7 | 72.4 ± 13.5a | 102.4 ± 17.2a |
20. Central amygdala | −2.80 | 2.9 ± 0.7 | 25.5 ± 3.2a | 20.6 ± 3.3a |
35. Lateral parabrachial nucleus | −9.30 | 2.6 ± 1.3 | 18.0 ± 0.6a | 13.1 ± 2.0a |
37. Nucleus of the solitary tract | −13.68 | 1.5 ± 0.7 | 39.3 ± 5.3a | 32.6 ± 6.2a |
Areas with significant WAY 267,464-induced c-Fos expression above oxytocin and / or vehicle |
||||
1. Medial prefrontal cortex / Prelimbic cortex | +2.70 | 9.5 ± 2.0 | 3.9 ± 1.4 | 12.0 ± 2.8b |
12. Median preoptic nucleus | −0.26 | 4.9 ± 1.0 | 8.4 ± 1.2 | 11.9 ± 2.2a |
16. Supraoptic nucleus (× 40) | −1.30 | 4.6 ± 2.6 | 11.0 ± 4.0 | 41.5 ± 9.3a,b |
27. Medial amygdala (posterodorsal) | −3.14 | 6.0 ± 1.5 | 6.0 ± 1.7 | 15.8 ± 1.7b |
34. Periaqueductal grey (ventrolateral) | −8.72 | 3.4 ± 1.0 | 6.9 ± 1.4 | 9.4 ± 1.8a |
Areas with significant oxytocin-induced c-Fos expression above WAY 267,464 and / or vehicle |
||||
36. Locus coeruleus | −9.68 | 0.1 ± 0.1 | 5.9 ± 1.3a,c | 1.6 ± 0.8 |
Areas with significant oxytocin-induced c-Fos expression below WAY 267,464 and / or vehicle |
||||
26. Lateral habenula | −3.14 | 6.5 ± 1.8 | 1.1 ± 0.6a,c | 6.3 ± 1.3 |
Areas with significant oxytocin-induced c-Fos expression above vehicle | ||||
11. Bed nucleus of the stria terminalis (dorsolateral nucleus) (× 40) | −0.26 | 3.1 ± 1.0 | 10.8 ± 2.4a | 6.1 ± 1.1 |
Significantly different from vehicle;
Significantly different from oxytocin;
Significantly different from WAY 267,464. All counts were conducted at × 20 magnification, unless stated otherwise (× 40).