Table 5.
Mean ± SEM number and proportion of c-Fos and oxytocin-labelled cells in the supraoptic nucleus (SON) and paraventricular hypothalamic nucleus (PVN).
| Region | Vehicle (n = 8) |
1 mg/ kg Oxytocin (n = 8) |
100 mg / kg WAY 267,464 (n = 8) |
|---|---|---|---|
| Supraoptic nucleus (× 40) | |||
| c-Fos only (n) | 1.3 ± 0.6 | 1.6 ± 1.0 | 4.1 ± 1.2 |
| c-Fos + oxytocin (n) | 8.8 ± 3.6 | 10.8 ± 4.7 | 36.7 ± 7.1a,b |
| Oxytocin-labelled cells (n) | 49.4 ± 4.2 | 45.4 ± 4.7 | 60.2 ± 4.4 |
| Percentage double-labelled (%) |
16.5 ± 6.4 | 20.8 ± 7.1 | 59.8 ± 10.8a,b |
| Paraventricular hypothalamic nucleus | |||
| c-Fos only (n) | 11.2 ± 3.4 | 50.7 ± 9.0a | 67.7 ± 9.9a |
| c-Fos + oxytocin (n) | 2.9 ± 1.0 | 21.2 ± 4.6a | 50.2 ± 8.4a |
| Oxytocin-labelled cells (n) | 55.6 ± 2.7 | 65.5 ± 6.6 | 70.4 ± 2.7 |
| Percentage double-labelled (%) |
5.4 ± 2.1 | 31.2 ± 6.4a | 70.0 ± 10.9a,b |
a
Significantly different from vehicle;
b
Significantly different from oxytocin.
All counts were conducted at × 20 magnification, unless stated otherwise (× 40).