Fig. 1.

Fml1-Mhf is required for wild-type levels of NCO during meiosis. (A) Schematic of the meiotic recombination assay indicating the positions (in base pairs) of ura4⁺-aim2 (green), his3⁺-aim (blue) and ade6 (yellow) on chromosome 3. The point mutations in the ade6-3083/-M26 hotspot and ade6-469 coldspot alleles are labelled in red and light blue, respectively. The common types of outcomes of the assay are shown: (I) GC at ade6 without CO, (II) GC at ade6 with CO of the flanking markers, and (III) CO without GC at ade6. (B and C) Frequency of CO associated with GC events at ade6 hotspots in wild type and mutants (tables S1 and S2) (2). (D) Frequency of CO in two neighbouring intervals in wild type and the fml1Δ mutant (table S4). In (B) to (D), statistical significance in comparison with wild type indicated as *P <0.1, **P <0.05, and ***P <0.01 (for P values, see corresponding tables in the supplementary materials). (E) D loop unwinding by Fml1ΔC (lanes b to d: 0.05 nM, 0.5 nM, and 5 nM), Fml1ΔC-K99R (lane e: 5 nM) and Fml1ΔC-D196N (lane f: 5 nM). The schematics represent the D loop and its dissociation products, with the asterisk indicating the position of the 5′ end ³²P label.