Figure 4. Effect of NaHS on CX3CL1 expression, IκBα content and p65 DNA binding activity in RAW264.7 cells stimulated with IFN-γ or LPS.

RT-PCR analysis for CX3CL1 mRNA (A), ELISA for lysate CX3CL1 protein level (B), western blot analysis for IκBα content (C) and ELISA for p65 DNA binding activity in nuclear extracts (D) were carried out as described in Materials and Methods. The data are means ± SEM of at least three independent experiments. *P<0.05, compared with unstimulated cells (control). ‡P<0.05, compared with stimulated cell pretreated with saline.