Table 6. PCR primer sequences, optimal conditions and product sizes.

Gene	Primer sequence	Optimal conditions	Size
CSE	Forward:5′-GAC CTC AAT AGT CGG CTT CGT TTC-3′Reverse:5′-CAG TTC TGC GTA TGC TCC GTA ATG-3′	34cyclesAnnealing: 61°C	618 bp
CX3CR1	Forward:5′-CCT GCC TCT GAG AAA TGG AG -3′Reverse: 5′- ATC TCT CCA GCC CCT GAA AT -3′	36 cyclesAnnealing: 58°C	332 bp
CX3CL1	Forward:5′-ATG ACC TCA CGA ATC CCA GTG -3Reverse: 5′-CCG CCT CAA AAC TTC CAA TGC -3′	36 cyclesAnnealing: 60°C	453
CCR2	Forward: 5′-AGAGAGCTGCAGCAAAAAGG-3′Reverse: 5′-GGAAAGAGGCAGTTGCAAAG-3	35 cyclesAnnealing: 56°C	185 bp
CCR5	Forward: 5′-CGAAAACACATGGTCAAACG-3′Reverse: 5′- CATGGCACCTGCCTCAAGTCTC-3′	36 cyclesAnnealing: 52°C	364 bp
GAPDH	Forward: 5′-GCA CAG TCA AGG CCG AGA AT -3′Reverse: 5′-GCC TTC TCC ATG GTG GTG AA -3′	22cyclesAnnealing: 54°C	151 bp