Figure 5. Analysis of virus yield in the presence of 2′OMe AONs complementary to domain 3.

(A) FMDV RNA was annealed with the indicated AONs or a scramble (SCR), prior to transfect confluent BHK-21 cell monolayers in duplicate. Virus yield was determined as PFU/ml in the supernatant 24 hpt, and made relative to the value obtained in the control RNA alone. Values represent the mean and standard deviation of triplicate assays (*** P<0.01; ** P<0.05: * P<0.1). (B). Western blot of representative examples of viral protein VP1 accumulated in BHK-21cells 24 hpt; anti-tubulin was used as a loading control.