Table 3. RNAi-based DtSER-1 deorphanization cAMP raw values.
| EXP | Treatment | Control | DtSER-1 RNAi | Sig | Δ cAMP stimulation |
| 1 | C | 29.43±1.26 | 30.43±2.56 | ns | |
| 5-HT | 153.29±4.89 | 99.18±2.26 | *** | −37% | |
| 2 | C | 129.33±4.57 | 114.29±13.22 | ns | |
| 5-HT | 498.56±39.86 | 324.32±42.00 | ** | −26% | |
| 3 | C | 14.71±0.91 | 18.02±1.040 | ns | |
| 5-HT | 113.18±4.42 | 96.42±1.69 | *** | −31% |
RIA-determined cAMP values (pM) are provided for three separate experiments (mean 
SEM). Treatments: C (control) and 5-HT (serotonin). Analysis (one-way ANOVA, Tukey) of these raw datasets establishes a significant decrease in 5-HT mediated cAMP stimulation associated with DtSER-1 suppression. For each experiment, Control groups and 5-HT treated groups are compared between Control RNAi and DtSER-1 RNAi conditions. Asterisks indicate significance at P
0.001 (***), P
0.01 (**), and “ns” means no significant difference.