Figure 5. Co-administration of AT2220 promotes greater tissue glycogen reduction in GAA KO mice.

Twelve-week old male GAA KO mice were administered vehicle (water) or AT2220 (10 or 30 mg/kg) via oral gavage once every other week for 8 weeks. Thirty minutes after each AT2220 administration, vehicle (saline) or rhGAA (20 mg/kg) was administered via bolus tail vein injection. Mice were euthanized 21 days after the last (i.e., 4^th) rhGAA administration and tissue glycogen levels were measured as described in ‘Materials and Methods’. Dotted lines show glycogen levels in the respective tissues of wild-type C57BL/6 mice. The data presented are an average of two independent studies with each bar representing the mean±SEM of the activity measured from 12 mice per group. Statistically significant reductions were seen in glycogen levels compared to baseline (*p<0.05, t-test) and compared to rhGAA administration alone (#p<0.05, t-test). In addition, the effect of AT2220 co-administration was also found to be significant for a linear trend (p<0.05; except in triceps), indicating a dose-dependent effect.