Figure 6. Co-administration of AT2220 promotes cell type-specific reduction of glycogen in GAA KO mice.

Twelve-week old male GAA KO mice were administered vehicle (water) or AT2220 (30 mg/kg) via oral gavage once every other week for 8 weeks. Thirty minutes after each AT2220 oral administration, vehicle (saline) or rhGAA (20 mg/kg) was administered via bolus tail vein injection. Mice were euthanized 21 days after the last (i.e., 4^th) rhGAA administration and glycogen levels in heart and quadriceps were measured immunohistochemically as described in ‘Materials and Methods’. A strong glycogen signal, represented as dark blue or pink spots (denoted with arrows) in heart and quadriceps, respectively, was observed. (*) indicates glycogen reduction in individual skeletal muscle fibers of quadriceps. The data shown are representative photomicrographs from 6 mice/group (magnification: 20X).