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. 2012 Jul 18;7(7):e40759. doi: 10.1371/journal.pone.0040759

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Macdonald et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Antigen solutions (50 nM) with and without imidazole (25 mM) were used to coat plates, and AAbs in cohort 1 serum samples were detected by ELISA (HTPA): a) an example of a concordance plot between the OD signal for p53 BirA with and without imidazole; b) the gradient (m) and fit (R²) values for the plot shown and the remaining cohort 1 data.