Figure 4. Anti-His Batch concordance.

Antigen solutions of Commercial and HTPE batches at 50 nM (outline) and 100 nM (filled) were used to coat plates, and proteins were detected using anti-his antibody by ELISA (HTPA) for five antigens: CAGE BirA (grey circle), Annexin I BirA (grey square), Cathepsin D BirA (black square), LMYC2 (black diamond) and Mesothelin BirA (black triangle). a) Concordance plot between the OD signal for all antigens at both concentrations; b) the gradient (m) and fit (R²) values for the plot shown with and without Mesothelin. The concordance for this antigen was poor, demonstrated by the fact that when the values are taken out a much greater concordance is observed for the remaining four antigens.