Table 4. Comparison of characteristics for HTP and previous R&D strategies.
| R&D Step | Characteristics | Previous strategy | HTP strategy |
| Cloning | Cloning type: | Restriction site | LIC |
| Number of constructs: | 2–3 | 1–96 | |
| Number of researchers: | 1 | 1 | |
| Timescale * (weeks): | 2–3 | 2–3 | |
| Cost per TAA ∼ (£): | 210 | 180 | |
| Expression | Batch type: | R&D | HTPE |
| Number of conditions: | 1∧ | 4“ | |
| Number of batches: | 7 | 192† | |
| Number of researchers: | 1 | 1 | |
| Timescale’ (weeks): | 1 | 1 | |
| Cost per TAA ∼ (£): | 40 | 6 | |
| Purification | Batch type: | R&D | HTPE |
| Number of batches: | 4 | 192† | |
| Number of researchers: | 2 | 1 | |
| Timescale (weeks): | 2 | 1 | |
| Cost per TAA ∼ (£): | 130 | 20 | |
| Assay | Assay type: | R&D 5 point assay | HTPA 2 point assay |
| Number of TAA tested: | 7 | 21 | |
| Number of researchers: | 4 | 4 | |
| Samples per day: | 200 | 200 | |
| Cost per TAA ∼ (£): | 108 | 36 |
*
Experimental timescale. Clonal verification by sequencing results and analysis may take longer or require additional man power. ∼ Approximation of consumable costs.
∧
More flasks or different conditions may be required if yields are poor. “Over 90% of the constructs tested so far are expressed under the HTPE screen conditions. †48 different proteins, 4 batches of each.‘Protein batches large enough for HTPA of up to 400 samples.