Table 1. Box-Behnken experimental design for independent variables and their corresponding observed and predicted values of response.

Run	Cysteamine (mM)	Glutaraldehyde (% v/v)	FunctionalizedGraphene (µg)	Enzyme (µg)	Immobilization (%)
					Actual	Predicted
1	5	3	500	600	64.2	63.74
2	15	4.5	1000	600	38.4	39.71
3	10	3	1500	900	56.3	56.4
4	10	1.5	500	600	44.2	44.37
5	15	1.5	1000	600	41.5	41.15
6	10	1.5	1000	300	30.2	30.66
7	15	3	1000	900	65.3	64.08
8	10	1.5	1000	900	56.4	56.94
9	10	3	1000	600	72.5	72.76
10	10	1.5	1500	600	40.1	40.28
11	10	3	1500	300	63.2	62.86
12	15	3	500	600	44.4	44.61
13	10	3	1000	600	73.5	72.76
14	10	3	500	900	83.5	84.13
15	10	3	1000	600	73.1	72.76
16	5	3	1000	900	68.2	68.32
17	15	3	1000	300	39.1	38.4
18	5	4.5	1000	600	62.3	62.95
19	15	3	1500	600	54.2	54.92
20	10	4.5	1500	600	58.1	57.35
21	5	3	1500	600	53.2	53.26
22	10	4.5	1000	900	65.1	64.91
23	5	1.5	1000	600	36.4	35.38
24	10	4.5	500	600	54.2	53.43
25	10	3	1000	600	72.1	72.76
26	10	4.5	1000	300	49.1	48.83
27	10	3	500	300	35.1	35.3
28	10	3	1000	600	72.6	72.76
29	5	3	1000	300	51	51.63