Table 4. Kinetic parameters of soluble and immobilized enzyme.
| Soluble | Immobilized | |||
| ONPG | Lactose | ONPG | Lactose | |
| Optimum pH | 2.8 | 4.0 | 3.8 | 4.0 |
| Optimum temperature | 60°C | 60°C | 60°C | 60°C |
| Km | 1.73 mM | 10 mM | 1.28 mM | 5.78 mM |
| Ki | – | 2.44 mM | – | 3.89 mM |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.