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. Author manuscript; available in PMC: 2013 Aug 1.

Published in final edited form as: Eur J Clin Invest. 2012 Mar 13;42(8):848–854. doi: 10.1111/j.1365-2362.2012.02660.x

Effects of incubation time at room temperature and storage media on enzymatic activities. a) β-glucuronidase. b) β-glucosidase. PBS, phosphate buffered saline alone at pH7.0, else with protease inhibitor (+PI), or acid (pH5.0), or both. Activities of both enzymes were consistently highest with PBS alone at pH7.0. Activities of both enzymes fell steeply during 2–4 hours at room temperature, and they increased over 24–48 hours at room temperature, perhaps from bacterial proliferation.

Effects of incubation time at room temperature and storage media on enzymatic activities. a) β-glucuronidase. b) β-glucosidase. PBS, phosphate buffered saline alone at pH7.0, else with protease inhibitor (+PI), or acid (pH5.0), or both. Activities of both enzymes were consistently highest with PBS alone at pH7.0. Activities of both enzymes fell steeply during 2–4 hours at room temperature, and they increased over 24–48 hours at room temperature, perhaps from bacterial proliferation.