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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Shock. 2012 Aug;38(2):146–152. doi: 10.1097/SHK.0b013e31825ce0de

Figure 3. CpG-ODN prevents the decrease in myocardial Akt phosphorylation that occurs after trauma-hemorrhage.

Figure 3

CpG-ODN (10ug/30g body weight) was intraperitoneally administered one hour before T-H. The PI3K inhibitor, LY 294002 (LY; 1mg/30g body weight) or the ERK inhibitor, U0126 (312 ug/30 g body weight) was administered by intraperitoneal injection, 15 min prior to CpD-ODN administration. Sham surgical operation served as sham control. Sixty min after T-H, hearts were harvested and the cytosolic proteins were isolated. The levels of phospho-Akt/Akt (P-Akt/Akt) were examined by Western blot with specific antibodies. V: vehicle; C: CpG-ODN; L+C: LY+CpG-ODN; U+C: U0126+CpG-ODN. N=4–5 mice/group. *P < 0.05 vs. corresponding sham; #P < 0.05 vs. CpG-ODN treated T-H.