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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Hum Mutat. 2012 Jun 7;33(8):1275–1284. doi: 10.1002/humu.22107

Table 2.

Experimental solution composition

Standard ND96 0 Cl−/gluconate ND96 0 Cl−/Br− ND96 0 Cl−/I− ND96 0 Cl−/NO3− ND96 0 Cl−/SCN− ND96 Final Conc. (mM) ND96 with 33mM HCO3− Final conc. (mM)
NaCl Na.gluconate NaBr NaI NaNO3 NaSCN 96 NaCl 63
KCl K.gluconate KBr KI KNO3 KSCN 2 KCl 2
MgCl2 Mg.(gluconate)2 MgBr2 MgI2 Mg(NO3)2.6H2O Mg.(gluconate)2 1 MgCl2 1
CaCl2 Ca.(gluconate)2 CaBr2 CaI2 Ca(NO3)2.4H2O Ca(SCN)2 1.8 CaCl2 1.8
HEPES HEPES HEPES HEPES HEPES HEPES 5.0 HEPES 5.0
NaHCO3a 33
pH 7.5 7.5 7.5 7.5 7.5 7.5
Osm 200±2 200±2 200±2 200±2 200±2 200±2
a

Before adding NaHCO3, bring solution to 90% volume, and regulate pH to 7.500 by NaOH. Then add 33mM NaHCO3, stir, and bring to 100% volume. Solution was bubbled with 5% CO2 for 30–40 minutes before experiments.