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. 2012 May 29;31(14):3092–3103. doi: 10.1038/emboj.2012.153

Figure 4.

Figure 4

Depletion of FGFR2b or Ets2 in the epithelium inhibits invasion. (A) Depletion of FGFR2b in E6/7-HFKs was confirmed by qPCR and knockdown of FGFR2b did not affect the expression levels of FGFR2c. Error bars represent s.d. (B) FGFR2b depletion in the epithelium significantly inhibited the ability of E6/7-HFKs to invade in the presence of Rb-depleted fibroblasts. Error bars represent s.e. (C) Quantification of the frequency of invasion. Likewise when Ets2 is depleted in E6/7-HFKs (confirmed by western blot (D) and qPCR (E) Error bars represent s.d.). Rb-depleted fibroblasts did not induce invasion of Ets2-depleted epithelium (F, G). Error bars represent s.e. All experiments were conducted in triplicate with independently generated cell lines. * Indicates P<0.05 determined by Students t-test. Scale bars represent 100 μm.