Figure 3.

The cytosolic glutathione reductase system is the major determinant of E_GSH[IMS]. (A) Scheme of the enzymes analysed in these experiments. (B) Steady states of the cytosolic and mitochondrial sensors (Grx1-roGFP2, Su9-Grx1-roGFP2, b₂-Grx1-roGFP2) in yeast mutants of the glutathione system. Experiment was performed as described in Figure 1C. (C–F) Recovery kinetics after diamide shock on mutants of the glutathione reductase system. WT yeast cells were compared to cells from Δglr1 (C), Δglr1+GLR1 (D) Δglr1+cyto-GLR1 (E) or Δzwf1 (F) strains, respectively. Cells expressing Grx1-roGFP2, Su9-Grx1-roGFP2 and b₂-Grx1-roGFP2 were analysed as described in Figure 1F (growth on galactose as carbon source). Reported values are the mean of three independent experiments. Error bars are the means±s.d.