Figure 8.
EGSH[IMS] is a determinant of the Mia40 redox state. (A) Scheme depicting the hypothetical influence of the glutathione pool on Mia40 thus giving rise to a partially reduced Mia40 at steady state. (B) Protein levels in wild type, Δglr1, GalL-Erv1 and GalL-Erv1/Δglr1 cells. (C) Redox states of Mia40 in wild type, Δglr1, GalL-Erv1 and GalL-Erv1/Δglr1 cells. Cells were analysed as described in Figure 7B. Reported values are the mean of three independent experiments. Error bars are the means±s.d. (D) Scheme for the set-up of an oxidant recovery experiment. Yeast cells were incubated with 20 mM diamide for 5 min at 30°C under constant agitation, the oxidant was removed by washing twice with measurement buffer, and the recovery of the Mia40 redox state was assessed at different times using the NEM alkylation-based gel shift assay. (E) Oxidant recovery kinetics of Mia40 in GalL-Erv1 and GalL-Erv1/Δglr1 cells. Cells were analysed as described in (C). Reported values are the mean of two independent experiments. Figure source data can be found with the Supplementary data.