Fig. 2.

MiR-191 overexpression is sufficient to decrease HEKn proliferation and induce senescence. (A) 48 h after transfection of HEKn cells with either a scrambled control (Ctr), miR-191 or antimiR-191, cells were subjected to a 4 h BrdU-pulse, collected, PI stained and analyzed by flow cytometry. BrdU positive cells are indicated as S phase fluorescent populations and are assessed by PI staining of DNA content of 2n or 4n (fixed to values of 200 and 400 in the plots). (B) BrdU positive cells percentage (BrdU%) of each sample in A) is reported in the histogram. Values represent the average ± SD of three independent transfections. (C) SA-β-galactosidase staining of HEKn cells 48 h after transfection with either a scrambled control (Ctr), miR-191 or antimiR-191. (D) Relative SA-β-galactosidase positive cells quantified by blue cell counting/field (as fold over control). Values reported are average ± SD of three independent stains. ^∗p-Value < 0.01 by Student’s t test.