Reorganization of PAGFP-GPI induced by perturbations of plasma membrane evaluated by PC-PALM. (a) Local density of PAGFP-GPI in clusters under steady state and after perturbations. Error bars, s.e.m. (n = 91 for untreated, n = 60 for no cholesterol, n = 58 with cholesterol treatment, n = 58 with sphingomyelinase (SMase), n = 59 with STxB and n = 53 with cytochalasin B). (b) Distribution of PAGFP-GPI molecules per cluster. (c) Distribution of correlation lengths of PAGFP-GPI under steady state and upon perturbations.