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. 2012 Jul 19;6:26. doi: 10.3389/fnana.2012.00026

Figure 1.

Figure 1

Synthetic peptide used to produce the anti-NaX antibody. (A) Representation of the partial nucleotide sequence of the NaX-ID2–3. The nucleotide sequence corresponds to the nucleic acid targeted for RT-PCR experiment carried out with the primers MOU66 and MOU59. The sequence of the synthetic peptide used for the rabbit immunization is highlighted in gray. (B) 12% acrylamide SDS-PAGE analysis characterizes the purification of the NaX-GST fusion protein. Lanes contain non-induced crude lysate (1), 0.1 mM IPTG-induced crude lysate (2) and purified 49 kDa NaX-GST fusion protein (3). M, protein size marker.