Figure 5.
Distribution of NaX immunostaining in the vascular organ of the lamina terminalis of the mouse. (A) Schematic illustration of the vascular organ of the lamina terminalis (OVLT) in the ventral forebrain of the mouse. The OVLT appears in red for better visualization. ac, anterior commissure; CPu, caudate putamen; HDB, horizontal limb of the diagonal band; LV, lateral ventricle; MS, medial septum; 3V, third ventricle. (B) Representative picture of the fluorescent NaX immunostaining (green) was obtained from the mouse. The OVLT region is delimited by the dashed line. 3V, third ventricle. The cell type expressing NaX was identified using anti-NeuN, anti-GFAP, and anti-vimentin fluorescent immunostaining (red); scale bar: 50 μm. (C) High magnification pictures of the staining merge; scale bar: 20 μm. The arrow head points the top-left corner of the enlarged zone. NaX staining is only present in vimentin positive cells.