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. 2012 Jul 19;6:26. doi: 10.3389/fnana.2012.00026

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Copyright © 2012 Nehmé, Henry, Mouginot and Drolet.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc.

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Distribution of Na_X immunostaining in the subfornical organ of the rat. (A) Schematic illustration of the subfornical organ (SFO) in the rat brain. The SFO appears in red for better visualization. cc, corpus callosum; CPu, caudate putamen; fi, fimbria hippocampus; ic, internal capsule; LV, lateral ventricle; 3V, third ventricle. (B) Representative picture of the fluorescent Na_X immunostaining (green) was obtained from the rat. The cell type expressing Na_X was identified using anti-NeuN, anti-GFAP, and anti-vimentin fluorescent immunostaining (red); scale bars: 50 μm. (C) Inset representing high magnification area of the SFO; scale bar: 20 μm. The enlarged zone is pointed by the arrow head (top-left corner of the inset). Na_X staining is present in NeuN positive cells in the core of the SFO and in vimentin positive cells in the cell layer boarding the third ventricle.