Figure 8.

Na_X immunostaining was restricted to the median preoptic nucleus of the rat. (A1) Schematic illustration of the median preoptic nucleus (MnPO) of the rat. The nucleus appears in red for better visualization. Note that the ventral part is separated from the dorsal part of the nucleus by the anterior commissure (ac). f, fornix; HDB, horizontal limb of the diagonal band; ic, internal capsule; LV, lateral ventricle; ox, optic chiasm; 3V, third ventricle. (A2) Representative picture of fluorescent Na_X immunostaining (green) was obtained from the ventral part of the rat MnPO; scale bar: 50 μm. The cell type expressing Na_X was identified using anti-NeuN fluorescent immunostaining (red). The arrow head points the top-right corner of the inset that represents a high magnification zone of the ventral MnPO; scale bar: 20 μm. Note the colocalization of the fluorescent Na_X and NeuN immunostaining. (B1) Schematic illustration of the mouse MnPO (red filling). (B2) Representative picture showing that fluorescent Na_X immunostaining was restricted to the cell layer boarding the third ventricle in the mouse; scale bar: 50 μm. The arrow head points the top-right corner of the inset that represents a high magnification zone of the ventral MnPO in the rat (A3) and in the mouse (B3); scale bar: 20 μm.