Figure 2.

Dilution bioassay showing phenotypic responses of S. cerevisiae strains to amphotericin B (AMB). S. cerevisiae sod2Δ [mitochondrial superoxide dismutase (Mn-SOD) gene deletion] mutant exhibited increased sensitivity to AMB over that of other deletion mutants (yap1Δ, sod1Δ, and glr1Δ) involving antioxidation responses. Data are representative results shown from 2 μg mL⁻¹ (AMB). 1 × 10⁶ cells were serially diluted 10-fold in SG liquid medium, and were inoculated onto agar plates.