Table 2.
Chemosensitization of AMB by 2,5-DHBA*.
| Strains | Compounds | MIC alone | MIC combined | FICI | MFC alone | MFC combined | FFCI |
|---|---|---|---|---|---|---|---|
| C. albicans | 2,5-DHBA | 1.6 | 0.8 | 1.0 | 6.4 | 3.2 | 0.8 |
| ATCC 90028 | AMB | 2 | 1 | 4 | 1 | ||
| C. albicans | 2,5-DHBA | 1.6 | 0.8 | 1.0 | 6.4 | 3.2 | 1.0 |
| CAN276 | AMB | 2 | 1 | 2 | 1 | ||
| C. krusei | 2,5-DHBA | 3.2 | 3.2 | 2.0 | >6.4† | 6.4 | 0.8 |
| ATCC 6258 | AMB | 2 | 2 | 4 | 1 | ||
| C. krusei | 2,5-DHBA | 3.2 | 0.0125 | 0.5 | >6.4† | 6.4 | 1.0 |
| CAN75 | AMB | 4 | 2 | 4 | 2 (99.7% killing) | ||
| C. tropicalis | 2,5-DHBA | 3.2 | 1.6 | 1.0 | >6.4† | 3.2 | 0.8 |
| CAN286 | AMB | 2 | 1 | 4 | 2 | ||
| C. neoformans | 2,5-DHBA | 3.2 | 1.6 | 1.0 | 6.4 | 3.2 | 1.0 |
| CN24 | AMB | 2 | 1 | 2 | 1 | ||
| t-Test | 2,5-DHBA | P < 0.05 | P < 0.01 | ||||
| AMB | P < 0.05 | P < 0.005 |
MFCs are concentrations where ≥99.9% fungal death was achieved, except where noted in the table. Synergistic interactions are in bold (see Materials and Methods for calculations).
*2,5-DHBA, 2,5-dihydroxybenzaldehyde (mM); AMB, amphotericin B (μg mL−1); MIC, minimum inhibitory concentration; MFC, minimum fungicidal concentration; FICI, fractional inhibitory concentration indices; FFCI, fractional fungicidal concentration indices.
†Assays were conducted up to the highest concentration of 6.4 mM. For calculation purposes, 12.8 mM (doubling of 6.4 mM) was used.