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. Author manuscript; available in PMC: 2012 Jul 19.
Published in final edited form as: Stem Cells. 2009 Aug;27(8):1712–1721. doi: 10.1002/stem.101

Fig. 2.

Fig. 2

Over-expression of PDGF-D in PC3 cells up-regulates the expression of transcription factors and down-regulates expressions of epithelial specific genes. (A) Real time RT-PCR was used to determine mRNA levels of epithelial markers in PC3 Neo and PC3 PDGF-D cells. Relative mRNA levels were normalized to GAPDH. (B) Real time RT-PCR was used to quantify the expression of ZEB1, ZEB2 and vimentin mRNA in PC3 Neo and PC3 PDGF-D cells. GAPDH was used for internal control to correct for the potential variation in RNA loading. (C) The results from real time RT-PCR showed the increased mRNA levels of E-cadherin, F11R, CRB3, and sciellin in PC3 PDGF-D transfected with ZEB1 siRNA compared to cells transfeced with control siRNA. Relative mRNA levels were normalized to GAPDH. (D) The decreased mRNA levels of ZEB1, ZEB2 and vimentin were observed in PC3 PDGF-D transfected with ZEB1 siRNA compared to control siRNA. Relative mRNA levels were normalized to GAPDH. (E) PC3 PDGF-D cells were transfected with ZEB1 or control siRNA and incubated for 72 h. Western blot analysis was performed using primary antibodies against ZEB1 and GAPDH. GAPDH was used for protein loading control. *, p < 0.05, **, p < 0.01 compared to control cells. (Neo: PC3 Neo cells, PDGF-D: PC3 PDGF-D cells, Con: control, E-cad: E-cadherin, Vim: vimentin).