Table 2.
Summary of performance of multiplexed markers on Bio-Rad and Millipore
| Bio-Rad markers with acceptable performance a | Bio-Rad markers with <25% detectability b | Millipore markers with acceptable performance | Millipore markers with acceptable performance a | Millipore markers with < 25% detectability b |
|---|---|---|---|---|
| A2M CRP CTACK C_PEPTIDE EOTAXIN FERRITIN FGF_BASIC FIBRINOGEN GHRELIN GIP GLP_1 GLUCAGON GRO HAPTOGLOBIN HGF IFNG IL_16 IL_18 IL_1RA IL_2RA IL_6 IL_8 IL_9 INSULIN IP_10 LEPTIN MCP_1MC MIF MIG MIP_1B M_CSF PAI_1 PCT PDGF_BB RANTES RESISTIN SAA SAP SCF SCGF_B SDF_1A TNFA TRAIL VEGF VISFATIN |
B_NGF GM_CSF IFNA2 IL_12P40 IL_15 IL_1A IL_2 IL_3 LIF MCP_3 TNFB Bio-Rad markers with >20% CV c B_NGF GM_CSF G_CSF IL_10 IL_12P70 IL_13 IL_15 IL_17 IL_1A IL_1B IL_2 IL_4 IL_5 IL_7 LIF MCP_3 MIP_1A TNFB TPA |
AMYLIN_TOTAL BCA_1 CCL19_MIP3B CCL20_MIP3A CKINE CRP CTACK CXCL11_I_TAC CXCL6_GCP2 CXCL9_MIG C_PEPTIDE EGF ENA_78 EOTAXIN EOTAXIN_2 FGF_BASIC FIT_3_LIGAND FRACTALKINE GIP GLP_1 GLUCAGON GRO G_CSF IFNA2 IFNG IL_11 IL_12P40 IL_16 IL_1A IL_29_IFNG1 IL_33 IL_8 INSULIN IP_10 LEPTIN LIF MCP_1 MCP_2 MCP_3 MCP_4 MDC MIP_1A MIP_1B |
MIP_1D PP PYY SAA SAP SCD30 SCD40L SCF SDF_1A SEGFR SGP130 SILRII SIL_1RI SIL_2RA SIL_4R SIL_6R SRAGE STNFRI STNFRII SVEGFR1 SVEGFR2 SVEGFR3 TARC TNF-A TPO TRAIL TSLP VEGF |
EOTAXIN_3 GHRELIN IL_20 IL_21 IL_28A IL_3 IL_4 IL_5 M_CSF XCL1_LYMPHO Millipore markers with >20% CV c GM_CSF IL_10 IL_12P70 IL_13 IL_15 IL_17 IL_1B IL_1RA IL_2 IL_21 IL_23 IL_28A IL_3 IL_4 IL_5 IL_6_1 IL_7 IL_9 I_309 M_CSF TGFA TNFB XCL1_LYMPHO |
a
Acceptable performance was defined as: 1) being detectable in greater than 25% of the 100 samples on all three specimen types and 2) across-batch CVs of <20% for blinded duplicates placed on at least 2 of the three specimen types.
b
Markers with <25% detectability on at least one of three (serum, heparin plasma, and EDTA plasma) specimen types.
c
Markers with coefficients of variation (CVs) for across-batch duplicates >20% on 2 or more of 3 specimen types. CVs were calculated for 20 blinded duplicate samples for each specimen type that were placed across different batches.