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. 2012 Jun 26;95(3):356–365. doi: 10.1093/cvr/cvs194

Figure 4.

Figure 4

Antioxidants abolish PDGF-BB-induced JNK1 phosphorylation in Neo-PH cells. Quiescent cells were pretreated with either NAC or TEMPOL for 1 h and then stimulated with PDGF-BB (25 ng/mL) for 10 or 30 min. ERK1/2 and JNK1/2 phosphorylation levels were assessed by western immunoblot analysis in Neo-C (A and C) and Neo-PH (B and D). Phosphorylated bands were quantified and normalized to those of total ERK1/2 and JNK1/2. Values are mean ± SEM of three independent experiments using cells cultured from three different Neo-C and Neo-PH calves. *P< 0.01 vs. control data.