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. 2012 Jun 26;95(3):356–365. doi: 10.1093/cvr/cvs194

Figure 5.

Figure 5

U0126 and SP600125 attenuate Neo-PH cell proliferation. (A) Growth-arrested fibroblasts were pretreated with either U0126 or SP600125 for 1 h, stimulated with PDGF-BB (25 ng/mL) for 30 min, and then phosphorylation of ERK1/2 and c-jun was assessed by western immunoblot analysis. (B) Quiescent Neo-C and Neo-PH cells were pre-incubated for 1 h with either U0126 or SP600125, and then treated with PDGF-BB. Cell proliferation assay was performed after 48 h stimulation. Values are mean ± SEM of three independent experiments done with three different fibroblast populations cultured from three different control and hypoxia-exposed calves. *P< 0.01 vs. untreated Neo-C cells; **P< 0.01 vs. PDGF-BB-treated Neo-C cells; †P< 0.01 vs. untreated Neo-PH cells; ††P< 0.01 vs. PDGF-BB-treated Neo-PH cells.