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. 2012 Feb 16;6(8):1535–1543. doi: 10.1038/ismej.2012.4

Figure 4.

Figure 4

Interaction between R. bromii and E. rectale in co-culture. (a) Time course of total sugar utilization and reducing sugar release on YCFAS and M2S medium containing 0.2% boiled RS3 by monocultures and co-cultures of R. bromii L2-63 and E. rectale A1-86. (b) Comparison by qPCR, using primer pairs specific to each bacterium, of the relative growth of the two bacteria for the experiment shown in (a) in M2S medium. The signal obtained with each specific primer pair is expressed relative to the signal obtained with the ‘universal' primer pair. Equal volumes of the two monocultures were mixed immediately after inoculation (t0) or after 48 h growth (t48) confirming the greater growth of R. bromii relative to E. rectale on this substrate. The same analysis on the co-culture, however, showed that E. rectale had outgrown R. bromii after 48 h.