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. 2012 Mar 8;6(8):1586–1601. doi: 10.1038/ismej.2012.7

Table 1. Sample index for Saanich Inlet, Cariaco Basin and Framvaren Fjord microbial eukaryotes.

Sample ID Location Stationa,b Date (mm/yy) Latitude, longitude Description Oxytype Depth (m) O2 (mM)c H2S (μM) NO3− (μM) CH4 (nM) Size fraction (μ)A,B,C Sequences Library
1 Saanich Inlet SI03 02/06 48.35°N, 123.30°W Surface Oxic 10 212.0 0.0 26.7 123.0 2.7–0.2 324 SGPW
2 Saanich Inlet SI03 07/06 48.35°N, 123.30°W Surface Oxic 10 381.6 0.0 4.6 94.0 2.7–0.2 147 SGSO
3 Saanich Inlet SI03 11/06 48.35°N, 123.30°W Surface Oxic 10 249.0 0.0 25.5 47.7 2.7–0.2 283 SHAB
4 Saanich Inlet SI03 04/07 48.35°N, 123.30°W Surface Oxic 10 316.1 0.0 18.2 45.0 2.7–0.2 695 SHAW
5 Saanich Inlet SI03 04/08 48.35°N, 123.30°W Surface Oxic 10 263 0.0 19.2 28.8 2.7–0.2 ND ND
6 Saanich Inlet SI03 02/06 48.35°N, 123.30°W Oxic–anoxic interface Dysoxic 100 51.1 0.0 21.0 93.8 2.7–0.2 243 SGPZ
7 Saanich Inlet SI03 07/06 48.35°N, 123.30°W Oxic–anoxic interface Dysoxic 100 22.9 0.0 16.1 59.6 2.7–0.2 270 SGST
8 Saanich Inlet SI03 11/06 48.35°N, 123.30°W Oxic–anoxic interface Suboxic 100 15.4 0.0 13.0 38.2 2.7–0.2 360 SHAG
9 Saanich Inlet SI03 04/07 48.35°N, 123.30°W Oxic–anoxic interface Dysoxic 100 67.3 0.0 26.5 14.8 2.7–0.2 355 SHAZ
10 Saanich Inlet SI03 04/08 48.35°N, 123.30°W Oxic–anoxic interface Dysoxic 100 120.0 0.0 20.5 22.4 2.7–0.2 326 SHZW
11 Saanich Inlet SI03 02/06 48.35°N, 123.30°W Lower redox transition Suboxic 125 5.0 0.0 9.7 145.0 2.7–0.2 344 SGSC
12 Saanich Inlet SI03 07/06 48.35°N, 123.30°W Lower redox transition Suboxic 120 6.5 0.0 4.8 167.0 2.7–0.2 328 SGSX
13 Saanich Inlet SI03 11/06 48.35°N, 123.30°W Lower redox transition Suboxic 120 9.8 0.0 8.9 22.1 2.7–0.2 278 SHAN
14 Saanich Inlet SI03 04/07 48.35°N, 123.30°W Lower redox transition Dysoxic 120 26.9 0.0 20.6 9.0 2.7–0.2 267 SHBC
15 Saanich Inlet SI03 04/08 48.35°N, 123.30°W Lower redox transition Suboxic 120 18.1 0.0 13.2 7.1 2.7–0.2 336 SHZZ
16 Saanich Inlet SI03 02/06 48.35°N, 123.30°W Anoxic deep Anoxic 215 1.4 ND 1.8 734.0 2.7–0.2 345 SGSH
17 Saanich Inlet SI03 07/06 48.35°N, 123.30°W Anoxic deep Anoxic 200 0.0 ND 0.5 1273.8 2.7–0.2 343 SGTA
18 Saanich Inlet SI03 11/06 48.35°N, 123.30°W Anoxic deep Dysoxic 200 54 ND 19.8 19.9 2.7–0.2 339 SHAS
19 Saanich Inlet SI03 04/07 48.35°N, 123.30°W Anoxic deep Anoxic 200 1.1 5.6 0.0 857.8 2.7–0.2 334 SHBH
20 Saanich Inlet SI03 04/08 48.35°N, 123.30°W Anoxic deep Anoxic 200 0.0 2.1 0.1 708.3 2.7–0.2 375 SIAC
21 Cariaco Basin A 01/05 10.50°N, 64.66°W Above interface Suboxic 240 8.4 0.0     ⩾4.5 600  
22 Cariaco Basin A 05/05 10.50°N, 64.66°W Above interface Dysoxic 185 66.3 0.0     ⩾4.5 744  
23 Cariaco Basin BC 01/05 B;10.40°N, 64.46°W/C;10.40°N, 65.35°W Above interface Anoxic 260/240 0.0/3.1 4.0/0.0     ⩾4.5 356  
24 Cariaco Basin BC 05/05 B;10.40°N, 64.46°W/C;10.40°N, 65.35°W Above interface Suboxic 216/230 12.5/23.5 0.0/0.0     ⩾4.5 519  
25 Cariaco Basin A 01/05 10.50°N, 64.66°W Oxic–anoxic interface Anoxic 280 0.0 1.1     ⩾4.5 495  
26 Cariaco Basin A 05/05 10.50°N, 64.66°W Oxic–anoxic interface Suboxic 225 8.7 0.0     ⩾4.5 553  
27 Cariaco Basin BC 01/05 B;10.40°N, 64.46°W/C;10.40°N, 65.35°W Oxic–anoxic interface Anoxic 300/280 0.0/0.0 4.7/0.9     ⩾4.5 153  
28 Cariaco Basin BC 05/05 B;10.40°N, 64.46°W/C;10.40°N, 65.35°W Oxic–anoxic interface Anoxic 256/270 0.0/0.0 0.3/0.1     ⩾4.5 536  
29 Cariaco Basin A 01/05 10.50°N, 64.66°W Upper sulfidic boundary Anoxic 320 0.0 4.0     ⩾4.5 565  
30 Cariaco Basin A 05/05 10.50°N, 64.66°W Upper sulfidic boundary Anoxic 265 0.0 0.6     ⩾4.5 394  
31 Cariaco Basin BC 01/05 B;10.40°N, 64.46°W/C;10.40°N, 65.35°W Upper sulfidic boundary Anoxic 340/320 0.0/0.0 4.7/3.7     ⩾4.5 162  
32 Cariaco Basin BC 05/05 B;10.40°N, 64.46°W/C;10.40°N, 65.35°W Upper sulfidic boundary Anoxic 296/310 0.0/0.0 4.7/1.4     ⩾4.5 433  
33 Cariaco Basin A 01/05 10.50°N, 64.66°W Anoxic deep Anoxic 900 0.0 53.1     ⩾4.5 140  
34 Cariaco Basin A 05/05 10.50°N, 64.66°W Anoxic deep Anoxic 900 0.0 51.5     ⩾4.5 400  
35 Cariaco Basin BC 01/05 B;10.40°N, 64.46°W/C;10.40°N, 65.35°W Anoxic deep Anoxic 400/900 0.0/0.0 10.9/45.3     ⩾4.5 39  
36 Cariaco Basin BC 05/05 B;10.40°N, 64.46°W/C;10.40°N, 65.35°W Anoxic deep Anoxic 670/900 0.0/0.0 24.5/52.4     ⩾4.5 400  
37 Framvaren Fjord   05/04 58.09°N, 06.45°E Oxic–anoxic interface Anoxic 18 0.0 0.0     ⩾6.5 76 M-IF
38 Framvaren Fjord   11/04 58.09°N, 06.45°E Oxic–anoxic interface Anoxic 18 0.0 0.0     ⩾6.5 55 N-IF
39 Framvaren Fjord   09/05 58.09°N, 06.45°E Oxic–anoxic interface Oxic 18 100.0 0.0     ⩾6.5 127 S-IF
40 Framvaren Fjord   05/04 58.09°N, 06.45°E Upper sulfidic boundary Anoxic 20 0.0 180.0     ⩾6.5 74 M-A1
41 Framvaren Fjord   11/04 58.09°N, 06.45°E Upper sulfidic boundary Anoxic 20 0.0 470.0     ⩾6.5 79 N-A1
42 Framvaren Fjord   09/05 58.09°N, 06.45°E Upper sulfidic boundary Anoxic 20 0.0 20.0     ⩾6.5 42 S-A1
43 Framvaren Fjord   05/04 58.09°N, 06.45°E Anoxic intermediate Anoxic 36 0.0 600.0     ⩾6.5 52 M-A2
44 Framvaren Fjord   11/04 58.09°N, 06.45°E Anoxic intermediate Anoxic 36 0.0 470.0     ⩾6.5 54 N-A2
45 Framvaren Fjord   09/05 58.09°N, 06.45°E Anoxic intermediate Anoxic 36 0.0 670.0     ⩾6.5 108 S-A2

Abbreviations: CH4, methane; H2S, hydrogen sulfide; ND, not done; NO3−, nitrate; O2, oxygen.

a

Cariaco Basin samples from sites B and C were composited before sequencing based on related depth intervals with shared physical and chemical properties [ref].

b

Only full-length amplicon sequences from Framvaren Fjord sequences were deposited in Genbank [ref].

c

Limit of detection for oxygen sensor in Saanich Inlet is 3 μM.

A denotes GF/D prefilters (2.7 μm pore size) in-line with 0.22 mm Sterivex-GV filters (Millipore).

B denotes 47 mm Durapore membranes (0.45 mm pore size).

C denotes 47 mm Durapore membranes (0.65 mm pore size).

Oxytype ln [μM]O2; oxic >4.5, dysoxic 3.0–4.5, suboxic 0.5–3.0, anoxic <0.5.

Summary of eukaryotic SSU ribosomal RNA gene (18S rRNA) sequence data, as well as geochemical data, generated from the Saanich Inlet water column spanning the 2006–2008 time series.