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. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: J Neuropathol Exp Neurol. 2011 Sep;70(9):811–826. doi: 10.1097/NEN.0b013e31822c256d

Figure 5.

Figure 5

(A) Reactivity of antibodies used in this study with spastin isoforms. Human embryonic kidney (HEK) cells were infected with lentiviral vectors encoding full-length versions of human M1 and M87 spastin isoforms; lysates derived from these cells were probed with anti-spastin antibodies Sp6C6, Sp3G11/1 and Sp/AAA. All antibodies similarly recognized overexpressed M1 and M87 spastin isoforms. (B) Lysates of HEK cells overexpressing full-length M1 or M87 spastin as in Figure 5A were run along with lysates derived from T98G human glioblastoma cells, normal human astrocytes (NHA) and cultured cortical neurons (Neu). Samples were run on Bis-Tris SDS-PAGE to increase M1/M87 separation. Under this gel conditions, immunoblotting with Sp6C6 antibody indicates that T98G cells, NHA and cultured cortical neurons all predominantly express the spastin M87 isoform. A faint Sp6C6-immunoreactive band running at a slightly lower molecular weight than full-length M87 (asterisk) was observed in T98G cells. This band likely represents the M87 isoform without exon 4 (M87 Δ Ex. 4) (20) and was more prominent in NHA and cultured cortical neurons.