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. Author manuscript; available in PMC: 2012 Jul 19.
Published in final edited form as: Stem Cells. 2011 Feb;29(2):286–296. doi: 10.1002/stem.581

Figure 5.

Figure 5

Angiogenic effects of VEGFA treated and untreated hASC seeded scaffolds via cell autonomous and paracrine effects 7 days after surgery. A, Few hASC derived vessels were detected by immunohistochemistry for human-PECAM-1 (left column) in VEGFA treated hASCs, while no hASC derived vessels could be detected in all other groups. Immunohistochemistry for mouse-PECAM-1 (right column) revealed vessel formations in calvarial defects seeded with hASCs. Numerous mouse-PECAM-1 positive vessels were observed in calvarial defects seeded with VEGFA treated hASCs. Arrows indicate PECAM-1 positively stained vessels; arrowheads indicate erythrocytes. Scale bar: 50 μm. B, Quantification of the average vessel surface and C, average vessel count per 5x field. Statistical analysis was conducted utilizing the Mann-Whitney Test. P-values: **<0.005 and ***<0.0005.