Figure 7. Mgat1 mutant encapsulations break down over time.
L. clavipes attacked larvae were scored for the presence of a melanized capsule at 24–48 hours PA, allowed to age a further 24 hours, then scored again. (A) Proportion of w1118 (blue bars) and Mgat11/+ (green bars) capsules that remained intact at 48–72 hours PA. Error bars indicate standard error of the mean; * indicates p<0.01 relative to control. (B) Brightfield image of a Mgat11/+ larva with broken capsule pieces indicated by arrows. (C) All of the Mgat11/+ larvae with this phenotype contained live wasp larvae. (D,E) Brightfield and fluorescent images of a broken capsule piece from a msn-mCherry;Mgat11/+ larva. The piece was composed largely of mCherry positive lamellocytes. Scale bars indicate 0.5 mm in B, 100 µm in C and 10 µm in D,E.